Internal standards and response ratios

To standardize analyte responses, you can define two types of internal standards in the QUAN Review application:

  • Internal standards linked to analytes
  • Internal standards linked to other internal standards

Internal standards linked to analytes allow the calculation of a response ratio for the analyte. The analyte response is multiplied by the internal standard concentration and divided by the internal standard response.

In the second case (an internal standard linked to another internal standard), the secondary (linked) internal standard is used to calculate a response ratio for the primary internal standard. In this case, there are two options for calculating the response ratio for the analyte.

If Response ratio linked to secondary internal standard is turned off, the response ratio for the analyte is calculated as above, using only the primary internal standard. The secondary internal standard is then used only in the response ratio calculation for the primary internal standard.

If Response ratio linked to secondary internal standard is turned on, the analyte response is instead multiplied by the primary internal standard concentration and divided by the primary internal standard's response ratio (that is, the primary internal standard normalized to the secondary internal standard).