Adding internal standard processing information
To add internal standard processing information to your processing method:
- In the sidebar, click Internal Standards.
- On the Internal Standards page, select the Processing tab.
- To add internal standard processing information, use the settings described in the following table:
Parameter Description Smoothing To turn the smoothing parameters on or off, use the Smoothing toggle. Smoothing Width Smoothing width sets the smoothing range of data points.
To specify the smoothing width, in the Smoothing Width cell, specify the value.
Smoothing Iterations Smoothing iterations sets the number of times the smoothing function repeats. Increasing this parameter produces a more substantial smooth.
To specify the smoothing iterations, in the Smoothing Iterations cell, specify the value.
Thresholding Method Note: QUAN Review supports only absolute thresholding. If it is necessary to set a threshold, set this at an appropriate level for the running assay, for example, 15% of the LLMI of the assay (in line with CLSI guidance for blanks).Absolute turns the thresholding method on, and None means that no threshold method is selected.
To select between None and Absolute, use the Thresholding Method drop-down menu.
Peak Height Threshold Peak height is the distance from the bottom of the peak to its apex.
To specify the peak height, in the Peak Height Threshold cell, specify the value.
Peak Area Threshold Peak area is the total area under the peak.
To specify the peak area, in the Peak Area Threshold cell, specify the value.
Peak Selection Nearest selects the nearest peak to the retention time and Largest selects the largest peak in the retention time window.
To select between Nearest and Largest, use the Peak Selection drop-down menu.
RT Tolerance RT tolerance is the allowed tolerance between the detected and expected retention times.
To specify the RT tolerance, in the RT Tolerance cell, specify the value.
Confirmatory RT Tolerance Confirmatory RT tolerance is the allowed tolerance between the detected qualifier ion and the detected retention time of the quan ion.
To specify the confirmatory RT tolerance, in the Confirmatory RT Tolerance cell, specify the value.
Signal-to-noise This determines the calculation used for signal-to-noise. The RMS is the quadratic mean of the noise range selected. The peak-to-peak value is the difference between the greatest and the smallest signal in the noise range selected.
To select among Peak to Peak, RMS, and RMS-1, use the S/N method drop-down menu.
- To add a new row, click
, and then specify the details in the relevant cells.
- To delete a row, select the row, and then click
.
- To save your internal standard information, in the upper-right corner, click
.
- To discard the changes, click
.