Adding internal standard information
Requirement: Follow your organization's standard operating procedures when naming internal standards and groups.
To add internal standard information to your processing method:
- In the sidebar, click Internal Standards.
- On the Internal Standards page, select the Internal Standards tab.
- To add a new internal standard, click
.
- To add internal standard name, internal standard concentration, expected retention time, precursor mass, or product mass details, click the relevant cell and type the details.
- You can use internal standards to standardize analytes or other internal standards. Optionally, link a second internal standard from a different row of the table to an internal standard using the
Linked Internal Standard drop-down menu. You use the second internal standard to standardize the internal standard in the row.
Notes:
- This feature enables workflows with analytes and two types of standards. For more information, see Internal standards and response ratios.
- An internal standard cannot be linked to itself.
- You can only use each internal standard for one purpose. For example, you cannot link an internal standard already linked to an analyte to another internal standard, and it is not available in the Linked Internal Standard drop-down menu. Also, you cannot link an internal standard linked to another internal standard directly to an analyte, and it is not available when defining analyte information.
- To add an additional precursor mass, in the
Precursor Mass cell, click
.
- To add a new product mass, in the
Product Mass cell, click
.
- To add a new row, click
.
- To delete a row, select the row to delete, and then click
.
Note: To delete a precursor mass, click the mass of the precursor mass, click Delete precursor mass, and then click OK. - To group internal standards together, select the rows you want, click
, specify a group name, and then click Assign.
Note: To ungroup internal standards, select the group as above, and then click Clear. - To save your internal standard information, in the upper-right corner, click
.
- To discard the changes, click
.