Adding analyte processing information

To add analyte processing information to your processing method:

  1. In the sidebar, click Analytes.
  2. On the Analytes page, select the Processing tab.
  3. To add analyte processing information, use the settings described in the following table:
    Parameter Description
    Smoothing To turn the smoothing parameters on or off, use the Smoothing toggle.
    Smoothing Width

    Smoothing width sets the smoothing range of data points.

    To specify the smoothing width, in the Smoothing Width cell, specify the value.

    Smoothing Iterations

    Smoothing iterations sets the number of times the smoothing function repeats. Increasing this parameter produces a more substantial smooth.

    To specify the smoothing iterations, in the Smoothing Iterations cell, specify the value.

    Thresholding Method
    Note: QUAN Review supports only absolute thresholding. If it is necessary to set a threshold, set this at an appropriate level for the running assay, for example, 15% of the LLMI of the assay (in line with CLSI guidance for blanks).

    Absolute turns the thresholding method on, and None means that no threshold method is selected.

    To select between None and Absolute, use the Thresholding Method drop-down menu.

    Peak Height Threshold

    Peak height is the distance from the bottom of the peak to its apex.

    To specify the peak height, in the Peak Height Threshold cell, specify the value.

    Peak Area Threshold

    Peak area is the total area under the peak.

    To specify the peak area, in the Peak Area Threshold cell, specify the value.

    Peak Selection

    Nearest selects the nearest peak to the retention time and Largest selects the largest peak in the retention time window.

    To select between Nearest and Largest, use the Peak Selection drop-down menu.

    RT Tolerance

    RT tolerance is the allowed tolerance between the detected and expected retention times.

    To specify the RT tolerance, in the RT Tolerance cell, specify the value.

    Confirmatory RT Tolerance

    Confirmatory RT tolerance is the allowed tolerance between the detected qualifier ion and the detected retention time of the quan ion.

    To specify the confirmatory RT tolerance, in the Confirmatory RT Tolerance cell, specify the value.

    Fit Type

    Linear performs a linear regression and Quadratic performs a second-order regression.

    To select between Linear and Quadratic, use the Fit Type drop-down menu.

    Origin

    At the point of origin, response is zero and concentration is zero. Force makes the best-fit line pass through the 0,0 point, Exclude does not include this point in the regression, and Include treats the origin as a point in the regression.

    To select Force, Exclude, or Include, use the Origin drop-down menu.

    Weight Type

    Specify this parameter setting to weight calibration points that show a low concentration or response when you fit a calibration curve by regression.

    To select between 1/x and 1/x2, use the Weight Type drop-down menu.

    Signal-to-noise

    This determines the calculation used for the signal-to-noise calculation. The RMS is the quadratic mean of the noise range selected. The peak-to-peak value is the difference between the greatest and the smallest signal in the noise range selected.

    To select among Peak to Peak, RMS, and RMS-1, use the S/N method drop-down menu.

  4. To add a new row, click , and then specify the details in the relevant cells.
  5. To delete a row, select the row, and then click .
  6. To save your analyte information, in the upper-right corner, click .
  7. To discard the changes, click .