AccQ•Tag Column for HPLC/UHPLC

The AccQ•Tag Amino Acid Analysis Column is a high-efficiency column specifically certified for use with the AccQ•Tag method. This HPLC column separates the amino acid derivatives produced by the AccQ•Fluor derivatization reaction.

AccQ•Tag Derivitization Chemistry

The AccQ•Tag method uses pre-column derivatized reagents to separate and easily detect fluorescence adducts using traditional HPLC instrumentation. The AccQ•Fluor reagent (6-aminoquinolyl-N-hydroxysuccinimidyl carbamate) is used to transform primary and secondary amines in a simple, single-step reaction to yield highly stable, fluorescent adducts that are detected using traditional HPLC.

Pico•Tag Column for HPLC

The Pico•Tag Amino Acid Analysis Column is a high-efficiency column specifically certified for use with the Pico•Tag method. This HPLC column separates the amino acid derivatives produced by phenylisothiocyanate (PITC) derivitization.

Pico•Tag Derivitization Chemistry

The Pico•Tag method is applicable to many samples including protein hydrolysates, physiologic fluids, feeds, foods and pharmaceutical preparations. Pre-column derivatization relies on the coupling reaction of the well-known Edman Degradation, the reaction of phenylisothiocyanate (PITC) with both primary and secondary amino acids to form phenylthiocarbamyl (PTC) derivatives. The PTC-amino acid adducts are stable and easily separated by reversed-phase HPLC. A single product is formed for each amino acid.

The Pico•Tag workstation is required to perform the Pico•Tag methodology. The workstation and parts can be purchased through Eldex by clicking the following link:

• Pico•Tag workstation