Manual Options

AccQ•Tag Ultra and AccQ•Tag chemistries are the first step in enabling your laboratory to obtain accurate amino acid composition from protein/peptide hydrolysates, physiologic fluids, feeds, foods, pharmaceutical preparations, and many other sample matrices. Combined with traditional HPLC, UHPLC, or the Waters ACQUITY UPLC, you can now have a system that is customized to separate amino acids from protein hydrolysates, cell culture media, and food/feed samples to get enhanced resolution. The end result is reliability with precise qualitative and quantitative results.

AccQ•Tag Ultra and AccQ•Tag chemistry kits include:

• Pre-column derivatization reagents
• Dedicated reversed-phase columns QC tested for amino acid analysis
• Eluents and standards
• Documentation on methods for the analysis of these important biocompounds

Automation Options

AccQ•Tag Ultra Derivatization Automation Kit provides simplified tools to enhance high throughput amino acid automation via an easy-to-use, automation friendly format that maximizes workflow and accommodates residual volume. Compatible with Tecan, Hamilton, and Andrew Alliance systems, the AccQ•Tag Ultra Derivatization Automation Kit provides fast, accurate, and precise amino acid analyses, translating into cost and time savings for your lab. 

• Parallel high-throughput sample prep (process up to 96 samples at a time)
• Minimal user intervention
• Larger reagent volume to accommodate automation needs
• Starter kits include reagents, column, eluents, application standards and labware
• Scripts ready for multiple automated platforms
• Tecan, Hamilton, and Andrew Alliance

Let Andrew+ Pipette for You

One of the largest pain points in sample preparation is repetitive pipetting. Minimizing sources of contamination is also critical. The Andrew+ robotic pipetting platform helps alleviate those pain points and provides confidence and reliability when doing derivatization. 

Andrew+ Pipetting Robot provides:

• Flexibility in the number of samples run using AccQ•Tag Ultra Automation Sample Preparation Kit (32, 64, 96 sample)
• Verified protocols available in OneLab
• Compatible with additional connected devices
• Easy-to-use, no in-depth training required
• Self-guided installation, online troubleshooting, and help

Amino Acid Reference Standards

Waters offers reference standards for Amino Acid Analysis (AAA) required in many applications in pharmaceutical and food and feed industries. A variety of standards containing free amino acids are offered for qualitative and quantitative determination of amino acids, method development, and troubleshooting of the AccQ•Tag Ultra and AccQ•Tag methods.

Available standards include:

• Cell Culture Standard Kit
• Food & Feed Standard Kit
• Amino Acid Hydrolysates Standard
• Internal Standard (Nva)

AccQ•Tag Ultra and AccQ•Tag Derivitization Chemistry

This line of products is a comprehensive, fully tested set of reagents, columns, eluents, and methods.   

AccQ•Tag is optimized for use with HPLC/UHPLC.

AccQ•Tag Ultra is optimized for use with the UPLC Amino Acid Analysis Application Solution.

• Both methods are based on the AccQ•Tag pre-column derivatization method
• Primary and secondary amino acids are reacted with a single reagent (AccQ•Fluor) in a high-throughput batch process resulting in exceptionally stable derivatives
• AccQ•Tag Ultra provides high-resolution separations achieved using the AccQ•Tag Ultra UPLC column (1.7 µm particles) and mobile phases
• Derivatized amino acids resolved and quantified to sub-pmol levels with single wavelength UV detection

Application: Protein Characterization and Cell Culture

Whether you need to determine amino acid composition of a product and compare it to the gene sequence or it's natural counterpart or if you are downstream and monitoring cell culture media for critical nutrients to optimize expression efficiencies and make timely product harvesting decisions, the UPLC Amino Acid Analysis solutions provide one solution for rugged and reliable performance to help make correct decisions about the product throughout an organization.

• Confirm quality
• Demonstrate process consistency
• Meet production schedules to minimize costly delays

Application: Food and Feeds

Animal feeds pose several significant challenges for accurate amino acid analysis. Quantitation of amino acids released by hydrolysis is one important measure of nutritional value. A free amino acid profile can identify the origin of a food product, and in that way, detect adulteration. Free amino acids are also metabolic indicators that can be used to monitor and optimize processes such as fermentation.

• Avoid costly reformulations
• Ensure products meet label claims for nutritional composition
• Confidence that products meet the manufacturing specifications

Turnkey Solution

The UPLC Amino Acid Analysis solution is a total application solution optimized for accurate, reliable, and reproducible analysis of amino acids. Based on AccQ•Tag Ultra chemistry combined with Waters award-winning ACQUITY UPLC separation technology, the UPLC Amino Acid Analysis Application Solution is designed for protein characterization, cell culture monitoring, and the nutritional analysis of foods and feeds.

Features:

• ACQUITY UPLC System and tunable UV detector
• AccQ•Tag Ultra derivatization chemistries including column, reagents, and eluents
• Empower 2 pre-configured projects, methods, and report templates
• Full system and application-level support documentation
• Application-specific performance qualification