Amino Acids Testing

Waters offers a comprehensive range of analytical system solutions, software, and services for scientists. Liquid Chromatography. Mass Spectrometry. Waters is the leading provider of lab equipment, supplies and software for scientists across the world. Easily research and order everything your lab needs! Waters offers a comprehensive range of analytical system solutions, software, and services for scientists. Liquid Chromatography. Mass Spectrometry. Waters is the leading provider of lab equipment, supplies and software for scientists across the world. Easily research and order everything your lab needs!

Quantification of Amino Acids

Amino acids occur in feed, food and beverages either as free amino acids or bound as building blocks of protein. The analysis of amino acids can include a number of steps, including hydrolysis to release bound amino acids, derivatization, separation of individual amino acids, detection and quantification. Quantitation of amino acids released by hydrolysis is an important measure of nutritional value. Free amino acids can also be useful metabolic indicators for monitoring and optimizing processes, such as fermentation.

Waters offers a number of manual and automated solutions for amino acid testing using pre-column derivatization, ensuring amino acids can be easily analyzed, enabling laboratories to:

−Ensure products meet label claims for nutritional composition

−Confirm products meet manufacturing specifications

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Easy-to-Use Sample Derivatization Kits

The amino acid analysis solution is based on the well-understood and widely used Waters® AccQ•Tag™ pre-column derivatization chemistry. The derivatives are separated using reversed-phase AccQTag Columns on an ACQUITY UPLC System for optimum resolution and sensitivity.

Pre-column derivatization reagents

Food and feed amino acid standard containing 21 AAs

Internal Norvaline standard

Dedicated reversed-phase columns QC tested for amino acid analysis

Mobile phase eluents

Accurate and Reliable Separation and Detection

The AccQ•Tag Ultra Amino Acid Analysis Columns are manufactured in Waters ISO 9002-certified facilities under strict guidelines to assure the highest quality. Combined with Waters ACQUITY UPLC separation technology and Empower CDS, the amino acid analysis solution enables accurate, reliable, and reproducible analysis of amino acids for protein characterization and nutritional analysis of foods and feeds.

Amino Acid Automation with Andrew+ Robot

A significant manual process in sample preparation is pipetting. This is a repetitive task using significant laboratory staff time and can introduce a potential source of error. The automation of this step allows laboratories to improve efficiency and provide confidence and reliability when performing sample derivatization for amino acid analysis.

Supporting Sample Preparation

Hydrolysis is an important first step in the workflow for the analysis of bound amino acids. It allows for the release of amino acids that can then be derivatized and separated using the Waters AccQ•Tag chemistries, coupled to ACQUITY UPLC technology. The hydrolysis primer compiles useful hints and tips for sample hydrolysis and serves as a starting point for those who are performing amino acid analysis, as well as a resource for experienced analysts who are trying to resolve a related issue.