Determine the spatial localization of the components of samples more quickly and easily with both increased sensitivity and clarity using the high-performance sprayer. This is complemented by more efficient ion transfer with the heated transfer line, further enhancing the sensitivity and molecular range of the DESI technique, making it possible to visualize compounds at previously unobtainable levels.

Improvements in sprayer usability and reproducibility allow higher quality data acquisitions with minimum optimization, enabling the translation of compound visualization into new knowledge and applied action quickly and easily.

These combined technologies contribute to improvements in overall in lab productivity, accelerating the rate of image generation by limiting the time required to acquire data at each individual pixel point.

Figure 3 shows a DESI XS generated image of murine brain section, demonstrating the spatial distribution of three different lipids (red, green and blue). Different spatial distributions within the brain suggest they serve different molecular functions.

In Figure 4, A. displays DESI images of [M-H]- lipid species in mouse kidney tissue sections, showing 20 and 40 µm spatial resolution. In B., it is possible to see a zoomed-in view of the same region of the kidney.

The source design includes a partially sealed housing that increases environmental stability and reduces atmospheric interference during the sample analysis, compared to an open DESI source. It offers a secure environment for both the user and the sample, providing a potential barrier from solvent vapors and sample aerosols while protecting the sample under analysis from contamination.

Unlike previous top-down visualization, the source also provides a camera for viewing the side of the DESI sprayer and the sample. This helps the user to locate the optimal spraying position for the DESI analysis more easily.

Internal components of the source have been designed with robustness and usability in mind. The sprayer armature is precision manufactured with a spring loaded arm encompassing caged roller bearings to give precision and durability for large scale acquisitions.

Partnering with a leading microscope stage manufacturer, a cutting-edge stepper motor stage has been incorporated into the source which has been designed to utilize different holders, enabling examination of a wide range of specimen types. Its unique S curve acceleration algorithm allows fast, smooth positioning without vibration, reducing disruption to samples and promising focused, consistent imaging.

For the first time, the source boasts integrated electronics and gas handling, meaning gases and voltages are automatically shut off at the end of your analysis. This is not only more environmentally friendly, but also saves on the cost of MS imaging consumables and prolongs the life of the DESI sprayer.

Futureproofing laboratories’ evolving imaging needs with Waters’ next-generation DESI MS imaging solutions.

The DESI XS source gives access to a minimal sample preparation method to answer easily, quickly, and accurately, “where is this component in my sample?”.

Access scalable imaging across the Waters MS portfolio, including the SELECT SERIES™ MRT, SELECT SERIES™ Cyclic IMS, SYNAPT™ XS, and Xevo™ G2-XS QToF.