Glycan Performance Test Standard

The Glycan Performance Test Standard line is intended to be used as a benchmarking standard for ACQUITY UPLC BEH Glycan, 1.7 µm Columns. 

• Two versions of labeled standards for 2‑AB (2‑aminobenzamide and RapiFluor-MS
• Human IgG based
• QC verified to contain the components needed to benchmark and evaluate HILIC columns

Dextran Calibration Ladder

In order to take full advantage of the BEH glycan column, Waters created a line of labeled Dextran Calibration Ladder Standards to assist glycan profiling. Glycan profile obtained from UPLC-HILIC/FLR systems can be calibrated against the labeled dextran ladder and assigned with glucose unit (GU) values. Waters Labeled Dextran Calibration Ladders are different than the existing commercial offerings. The average molecular weight of the glucose homopolymer is higher (~4,500 Dalton), therefore, the workable GU value range is twice as much as the other dextran ladder standards; the observed GU goes from 2 to 30. This feature improves the large glycan retention time assignment.

• Labeled standards for 2‑AA (anthranilic acid), 2‑AB (2‑aminobenzamide), and RapiFluor-MS
• Larger molecular weight range than typical commercially available ladders

The HILIC Advantage:

Hydrophilic Interaction Chromatography (HILIC) can be used to successfully improve retention of very polar species.  This technique is rapidly growing for this application area.  One reason for this is that this mode of separation is well suited for glycans due to their highly polar nature.  The GlycoWorks HILIC SPE provides an efficient and reproducible mechanism for effective glycan cleanup and provides quantitative and consistent recoveries for a diverse range of N-glycans.  Along with the GlycoWorks SPE Reagent Kit with a customized elution buffer this provides a very reproducible cleanup capturing species up to tetrasialyated.

• Sample clean-up devices with HILIC (amino-propyle) sorbent
  • High-throughput: GlycoWorks HILIC µElution SPE Plate (96-well)
  • Single Use: GlycoWorks HILIC 1 cc (20 pack) and GlycoWorks HILIC 1 cc Flangeless Cartridges for the positive pressure manifold (20 pack)

GlycoWorks 2-AB N-Glycan Kits

The GlycoWorks Reagent Kit has been updated to include material for protein deglycosylation and complete 2-AB labeling as a cost effective alternative.

Watch this video for step-by-step direction on how to perform glycan analysis using the GlycoWorks Kit.