LC-MS Enables Cutting Edge SARS-CoV-2 Research

The Waters SARS-CoV-2 LC-MS Kit (RUO) is a Research Use Only kit intended for the quantitative measurement of three (3) proteolytic peptides derived from the SARS-CoV-2 Nucleocapsid (NCAP) protein. The method has been optimized on an ACQUITY UPLC I-Class Plus and Xevo TQ-XS System, and is also intended for use with the Andrew+ Pipetting Robot. Optimization on other instrument platforms is possible.  

Further investigation is needed to collect objective evidence in promising research applications, however, based on the properties of the analytical test method, it might be leveraged for the following research studies:  

• Peptide concentration – quantitate SARS CoV-2 signature peptide at different stages of infection 
• Research of infection and long-term effects through peptide quantitation 
• Future pandemic preparedness studies and research of signature peptide-based biomarkers unique to other infections  

Confidence in Results

Ensure accurate and consistent results with highly analytically selective antibody magnetic bead enrichment.  The SARS CoV-2 LC-MS Kit (RUO) uses the innovative SISCAPA® anti-peptide antibody enrichment sample preparation method from SISCAPA Assay Technologies, Inc. that improves the performance of mass spectrometry for measurement of pre-selected protein targets. Achieve the analytical sensitivity required to detect low levels of SARS-CoV-2 peptides from complex digested samples with stable isotope standards and capture by anti-peptide antibodies. Increase laboratory efficiency as all components necessary to quickly deploy the supplied method are packaged together for the busy research laboratory.

• Highly analytically selective to three SARS-CoV-2 NCAP signature peptides 
• Lower limit of quantitation (LloQ) of 3amol/µL, as demonstrated in the accompanying application note 
• Anti-peptide antibody capture results in clean, low-noise chromatograms with an ultra-fast run time (<2 minutes) 
• The method minimizes sample media interference, providing greater flexibility for the analysis of multiple research samples

SISCAPA® is a mark of Anderson Forschung Group LLC (AFG).

Trusted Chemistries and Consumables

Ensuring reproducible quantitation from sample-to-sample, run-to-run, and lab-to-lab provides the confidence needed for making critical research decisions. Variability risks can be reduced by minimizing the risk of unpredictable non-specific binding of peptides to surfaces. The SARS-CoV-2 LC-MS Kit employs QuanRecovery Plates with MaxPeak HPS and a MaxPeak Premier Peptide Column which may:

• Minimize non-specific binding of peptides to surfaces
• Enhance recovery and signal
• Increase reproducibility
• Improve peak shapes

Automation

Save valuable time, allowing you to focus on your research, with reliable and reproducible sample preparation using the Andrew+ Pipetting Robot. The SARS-CoV-2 LC-MS Kit (RUO) comes in an automation-friendly format with liquid handling protocols for the Andrew+ Pipetting Robot available on OneLab.

• Consumables compatible with automation
• Flexible automation platform easily adaptable to multiple workflows
• SARS-CoV-2 LC-MS Kit (RUO) automation protocol available for Andrew + Pipetting Robot