Oasis Sample Extraction Products

Oasissample extraction products deliver unmatched purity, consistency, and quality for sample analysis. With a broad range of sorbents and an array of formats, Oasis products meet a wide variety of sample preparation needs, from simple and fast sample matrix cleanup to more complex sample preparation challenges. Oasis polymeric products deliver consistent retention and capacity, even if the sorbent dries out. Oasis products deliver:

Optimal sensitivity for small molecule and therapeutic peptide analysis
Reduced matrix effects
Unsurpassed selectivity
Consistent retention and capacity, yielding the highest SPE recovery

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Oasis Solid-Phase Extraction (SPE) Sorbents

Oasis sorbents are available in seven different solid-phase extraction (SPE) chemistries, providing a range of options for method development.

Oasis PRiME HLB is an all purpose, strongly hydrophilic, reversed-phase, water-wettable polymer with a unique Hydrophilic-Lipophilic Balance. Oasis PRiME HLB sorbent is designed to simplify solid-phase extraction (SPE) by taking advantage of the desirable water-wettable and retention characteristics of Oasis HLB while providing cleaner samples in fewer steps, due to simpler protocols and the elimination of sorbent condition and equilibration steps. Oasis PRiME HLB is ideal for all compound classes.

NEW OASIS PRIME MCX combines the simplicity and cleanliness of Oasis PRiME HLB with the specificity of a cation-exchanger for compounds with basic characteristics, and provides the perfect solution for targeted sample clean-up.

Oasis HLB is an all purpose, strongly hydrophilic, reversed-phase, water-wettable polymer with a unique Hydrophilic-Lipophilic Balance. Oasis HLB maintains high retention and capacity even if it runs dry after conditioning. This sorbent is ideal for acidic, basic and neutral analytes as it's stable from pH 0-14.

Oasis MCX is a Mixed-mode, strong Cation-eXchange, reversed-phase, water-wettable polymer. Oasis MCX is selective for bases and stable in organic solvents.

Oasis MAX is a Mixed-mode, strong Anion-eXchange, reversed-phase, water-wettable polymer. Oasis MAX is selective for acids and stable in organic solvents.

Oasis WCX is a mixed-mode, Weak Cation-eXchange, reversed-phase, water-wettable polymer. Oasis WCX is used to retain and release strong bases and is stable in organic solvents.

Oasis WAX is a mixed-mode, Weak Anion-eXchange, reversed-phase, water-wettable polymer. Oasis WAX is used to retain and release strong acids and is stable in organic solvents.

Improved Sample Preparation with Oasis SPE

Sample preparation is often necessary to improve various aspects of chromatography and mass spectrometry analyses. Solid-phase extraction (SPE) or technically known as "Liquid-Solid Phase Extraction" is a sample preparation technology that uses particles of chromatographic packing material, usually in a cartridge or 96-well plate format, to chemically separate different analytes and aid in removing the matrix components in a sample. There are some major benefits to using solid-phase extraction.

Clean-up of complex sample matrix with compound purification. It's a challenge for analytical chemists when compounds are contained in a complex sample matrix. There are many interfering substances in sample matrices. Solid-phase extraction provides easy sample clean up of plasma, urine, whole blood, serum and tissue matrices containing target drugs and any drug metabolites. SPE can also provide fast clean up of grains for mycotoxins analysis and also of shrimp for antibiotic residues. The benefits of dirty sample matrix cleanup with SPE include improved quantification while providing cleaner baselines to improve the accuracy of the analytical result.
Reduced ion suppression or enhancement in MS applications. Another challenge with complex sample matrices can be seen when looking at the analytical results (LC-MS or LC-MS/MS). For MS signal sensitivity, the compound ions must form properly. If ion suppression occurs due to interferences from a dirty sample matrix, the detection signal is significantly diminished. However, a simple SPE method could eliminate or greatly reduce these types of matrix effects.
Ability to fractionate compounds by class. When analyzing a sample with many types of compounds that need to be separated by class for further, more efficient analysis, a solid-phase extraction method can be developed to separate different classes of compounds, by, for example, polarity. These compounds can be collected as separate fractions from the non-polar compounds. Then the separate fractions can be analyzed more efficiently.
Trace concentration (enrichment) of very low level compounds. When analyzing samples containing compounds present at a low concentration levels (ie. parts per trillion (ppt), it may become difficult to achieve accurate and reproducible quantification of these analytes. SPE can provide sample enrichment in order to achieve significantly better results by concentrating larger volumes of sample into smaller elution fractions.

SPE is a proven sample preparation tool enabling simple sample matrix removal, including common interferences such as proteins, salts, fats and phospholipids. This matrix removal provides many benefits, including a reduction in matrix effects, increases in sample concentration and analyte specificity. It also significantly simplifies chromatographic complexity by reducing the number of interfering peaks in the LC as well as reducing signal suppression by providing clean samples for the MS.

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Oasis PRiME MCX

Traditional mixed-mode SPE protocols require six steps and still may not remove matrix interferences such as phospholipids. Oasis PRiME MCX is a highly efficient, orthogonal (reversed-phase and ion-exchange) solid-phase extraction product based on Oasis MCX technology. Sample clean-up is achieved with simple and fast three- and four-step protocols which remove up to 99% of phospholipids from complex sample matrices while providing high and reproducible target analyte recoveries. Oasis PRiME MCX is QC tested for performance using analyte recovery and phospholipid removal as benchmarks. In addition, cartridges and plates are designed with a manufacturing optimization to increase flow reproducibility across wells and devices, making sample processing time more predictable.　

Oasis PRiME MCX Advantages

SIMPLER: A defined, generic 3-or 4-Step SPE protocol based on the water wettable Oasis MCX strong cation-exchange / reversed-phase sorbent that does not require extensive method development. Allows targeted cleanup of basic compounds with pKa ≥ 4.5. Methods are patent pending　

CLEANER: Simpler methods remove up to 99% of phospholipids, a major cause of matrix effects, ion suppression, shorter column lifetimes, increased MS maintenance and higher variability in LC MS quantification. Oasis PRiME MCX is QC’d with this protocol for phospholipid removal

FASTER: Cartridges and plates are designed with a manufacturing optimization to increase flow reproducibility across devices, making processing time more predictable. No conditioning and equilibration steps are required. 　

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Oasis PRiME MCX 3-and 4-Step Protocols

Oasis PRiME MCX methods are designed to capture and concentrate basic compounds while matrix interferences are removed from the sample. The 3 step method provides the simplest path to cleaner, while the 4 step method contains an additional wash step to remove even more matrix components, if needed.

Oasis PRiME HLB

Oasis PRiME HLB is the first-of-its-kind, patent pending, solid-phase extraction (SPE) sorbent that sets the new performance standard for routine analyses. Based on the Oasis HLB Technology, the Oasis PRiME HLB Sorbent was developed to meet the needs of scientists looking for cleaner samples in less time and with less effort. Oasis PRiME HLB reversed-phase sorbent is designed to simplify SPE by taking advantage of the desirable water-wettable and retention characteristics of Oasis HLB while providing cleaner samples in fewer steps, due to simpler protocols and the elimination of sorbent condition and equilibration steps. This is the ideal choice for routine analysis of reversed-phase SPE of acidic, basic, and neutral analytes.

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Oasis PRiME HLB Advantages

SIMPLE three-and two-step protocols with no SPE expertise required
FASTER more even flows across 96-well plates and cartridges with less plugging
EVEN CLEANER samples with the removal of more than 95% of common matrix interferences such as proteins, salts, phospholipids, and pigments

Food Matrix Removal using Oasis PRiME HLB 2 Step Protocol

Additional benefits of using the 2-step pass-through protocol for multiresidue contaminant analysis:

No method development - The same pass-through protocol is applicable to various pesticides, veterinary drugs or natural toxins analyses

Time saving protocol - Used as the clean-up method following QuEChERS extraction. Saves significant time compared to traditional dispersive SPE method while achieving superior clean-up

Improve instrument up time - Cleaner samples lead to less dirty matrix injected into the system, resulting in longer instrument up time and less maintenance

Convenient and flexible formats – Traditional-style cartridges compatible with vacuum and positive pressure manifolds. "Plus" style cartridges can be used without manifolds when connected to a displacement syringe

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Oasis HLB methodology

Solid-phase extraction (SPE) method development starts with knowledge of the sample matrix—the nature and relative concentration of the analytes of interest and the potentially interfering endogenous compounds. The right sorbent and solvents are selected for load, wash, and elute steps, resulting in the creation of rugged, reliable, and selective protocols. Waters application chemists have devised several approaches to streamline this process.

The Oasis HLB method is a generic protocol used to isolate a broad range of acidic, basic, and neutral compounds from a wide spectrum of matrices.

Oasis 2x4 Method

The Oasis 2x4 method for selection of SPE sorbent and protocol for acids, bases, and neutrals produces the cleanest extracts by eliminating matrix interferences and sample variability. Two protocols and four sorbents provide the flexibility to extract acids, bases, and neutrals with high SPE recoveries while removing matrix components that may interfere with analysis. Follow the simple steps outlined in this flow chart to achieve high recoveries and the cleanest extracts:

Characterize the analyte (neutral, acid or base, pKa)
Select one of the four Oasis sorbents
Apply the indicated protocol (1 or 2)
Determine SPE recoveries by LC analysis

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Oasis Formats Available

Oasis products come in a full range of device formats to meet your SPE requirements and to overcome your SPE challenges.

Oasis μElution Plate	Oasis 96-Well Extraction Plates

Patented μElution plate design No evaporation and reconstitution necessary due to elution volumes as low as 25 µL Ideal for SPE cleanup and analyte enrichment of small sample volumes Up to a 15x increase in concentration Compatible with most liquid-handling robotic systems for automated, reliable high throughput SPE (HT-SPE)	Innovative award-winning two-stage well design High throughput and high recovery High throughput and high recovery Compatible with most liquid-handling robotic systems for automated, reliable high throughput SPE (HT-SPE)

Oasis Syringe-Barrel Cartridges	Oasis Glass Cartridges	Oasis On-Line Columns and Cartridges

Ultra-clean syringe barrel and frits Available in cartridges ranging from 1 cc to 60 cc Flangeless syringe-barrel cartridges available in 1 cc, 3 cc, and 6 cc sizes Plus-style cartridges with Luer inlet hub and outlet tip, compatible with hand-held syringes	Ultra-clean glass syringe with Teflon^® frit For trace level detection and analysis at part-per-trillion (PPT) levels Available in 5 cc (200 mg) configuration	For rugged, reproducible, and ultra-fast on-line analysis Wide choice of configurations, particle sizes, and sorbent chemistries Available with all six patented Oasis sorbents—PRiME HLB, HLB, MCX, MAX, WCX, and WAX High recovery and reproducible results for a wide range of compounds Cartridge format for use with Spark Holland Prospekt-2™/Symbiosis™ systems also available