This application note describes a rapid and simplified solid phase extraction protocol and LC-MS/MS method for the analysis of urinary benzodiazepines and metabolites.

The unique water wettable nature of the Oasis MCX Sorbent enables the elimination of the common conditioning and equilibration steps without any loss in recovery or reproducibility. This property of Oasis also enables the entire hydrolysis step to be conducted within the wells of the Oasis MCX μElution Plate, eliminating time consuming and error-prone transfer steps, reducing the total number of post-incubation steps from 9 to 5. Combining this extraction procedure with the chromatography of the CORTECS C₁₈+ Column, and the sensitive and reproducible quantification of the Xevo TQ-S micro mass spectrometer, results in a rapid and efficient analysis method that is also exceptionally accurate. This method is simpler, faster, and easier, than liquid-liquid extraction and cleaner than reversed-phase SPE while providing excellent sensitivity, accuracy and precision for the analysis of this important class of compounds.

View the Application Note