5 Rules of Scaling LC Purification: 1. Scale flow rate and sample load proportionately

Library Number:
LITR135132836
Part Number:
720008240EN
Author(s):
Jo-Ann Jablonski, Principal Scientist, Waters
Source:
Waters
Content Type:
Literature
Content Subtype:
Brochures

Scaling equations in preparative liquid chromatography are vital for maintaining resolution and chromatographic profile during scale-up, preventing peak broadening and changes in elution time. Consistent sample mass load across scales ensures uniform peak widths and resolution, crucial for reliable purification processes.


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