MaxPeak Premier SEC Peptide and Protein Columns
More confident biological compound separations start here.
Get superior size-based separations of biological compounds with MaxPeak Premier SEC Columns.
Size-exclusion chromatography (SEC) is a powerful technique for important size-based analyses of biotherapeutics peptides (insulin and GLP-1s) and proteins that include monoclonal antibodies (mAbs), antibody drug conjugates (ADCs), and bi-specifics.
See how our ACQUITY and XBridge Premier SEC columns help generate reproducible, high-resolution separations in the research, development, and commercialization of biotherapeutic products or diagnostic reagents.
MaxPeak Premier Protein SEC Columns
Now available: ACQUITY and XBridge Premier SEC 125 Å Columns
Our new ACQUITY and XBridge Premier SEC 125 Å Columns are ideal for biotherapeutic peptides and small proteins.
Explore all of Waters HPLC, UHPLC, and UPLC SEC column chemistries
Waters offers a wide variety of size-based separations for peptides, proteins, and viral vectors.
Specifications
Overview
ACQUITY & XBridge Premier SEC 125 Å Columns
- Up to 15% improved resolution
- 6x faster separations
- 7x solvent savings
- Reliable batch-to-batch consistency achieved through specific insulin QC batch testing
- Reduce secondary interactions with 2.5x increase in High Molecular Weight Products (HMWPs)
Recommended Use: For accurate HMWP, insulin monomer quantitation, GLP-1 Receptor Agonists, biotherapeutic peptides and small proteins from 1-80 KDa.
ACQUITY & XBridge Premier SEC 250 Å Columns
- Get 30% improved resolution with Waters MaxPeak Premier Protein SEC Columns
- Eliminate the need for extensive method development with a one-size-fits-all method in your lab
- Get unmatched batch-to-batch reproducibility with the wide range of Waters SEC columns and standards for peptide and protein characterization
- Limit unwanted secondary interactions that lead to broader peaks, inaccurate quantitation of aggregates, and increased tailing
Recommended Use: Platform method for protein size variant analysis from 10-650 KDa.
Features Header
MaxPeak Premier SEC 125 Å Columns
Performance benefits for insulin and GLP-1 size-based analyses
MaxPeak Premier SEC Columns with High Performance Surfaces (HPS) Technology leverage decades of Waters SEC column manufacturing and quality control testing expertise to provide better resolution and reduced secondary interactions for more accurate determination of HMWP species without the need for extensive column conditioning.
- Up to 15% improved resolution
- 6x faster separations
- 7x solvent savings
- QC tested with insulin for batch-to-batch consistency
- Achieve 2.5x increase in HWMPs and accurate insulin monomer quantitation for improved insulin analysis results
- High-throughput resolution on any HPLC, UHPLC and UPLC system
- Available in 1.7 µm (ACQUITY) and 2.5 µm (XBridge) particle sizes
- Reliable quantitation of covalent HMW GLP-1 aggregates using SEC-MALS under denaturing SEC conditions
MaxPeak Premier SEC 250 Å Columns
Performance benefits for GLP-1 receptor agonists size-based oligomer analyses
- Achieve excellent separation of GLP-1 using native SEC conditions
- Accurately monitor influence of SEC eluent pH and ionic strength on GLP-1s
Performance benefits for mAb, ADCs, bi-specifics
- Achieve 30% improved resolution with MaxPeak Premier Columns
- Get highly efficient separations to resolve species that vary by less than 2X in size on any LC
- Achieve narrow peak widths with reduced tailing for accurate quantitation of aggregates and fragments of <0.5% total peak areas and 30% better resolution
- Gain efficiency for proteins ranging from 10 KDa to 650 KDa, leveraging MaxPeak Premier Technology coupled with BEH-PEO particle technology
Selecting your MaxPeak Premier 125 Å and 250 Å Column
Developing an SEC-based separation begins by selecting the appropriate MaxPeak Premier 125 Å and 250 Å Column.
Longer columns (i.e., 300mm rather than 150mm) are recommended for increased component resolution. The most conservative SEC column offering for use on any UPLC, UHPLC, or HPLC system is an XBridge Premier SEC 125 Å or SEC 250 Å 2.5 um, 7.8 x 300 mm.
For more in-depth information and tips on SEC column chemistries, see our Guide to Size-Exclusion Chromatography.
More confident platform methods
- Eliminate the need for extensive method development by using a one-size- fits-all method in your lab
- Ensure accurate results, such as high molecular weight (HMW) percentage, which should not depend on mobile phase choice
- Resolve protein size variants for over 80% of proteins without the need for additives with a Phosphate Buffered Saline (PBS) eluent
Exceedingly inert for biologics
SEC should separate proteins based exclusively on size, however unwanted secondary interactions can lead to broader peaks, inaccurate quantitation of aggregates, and increased tailing. Waters has industry-leading innovations to limit secondary interactions on both column and particles:
- MaxPeak High Performance Surface columns mitigates ionic interactions between hardware and proteins
- Ethylene Bridged Hybrid (BEH) SEC particles with high coverage hydroxy-terminated polyethylene oxide (PEO) bonding minimizes hydrophobic particle interactions
- MaxPeak High Performance Surfaces enable out-of-the-box performance reducing the need for lengthy column conditioning typically observed in other columns
Stay compliant with smart column options
Ideal for compliant laboratories, smart column options are available for identifying and tracking column information. The eCord is a data recording device which is permanently attached to the column body of 2.x µm particle size (2.1 and 3.0 mm internal diameter) and ACQUITY UPLC columns and is standard for those column offerings. Rather, the eConnect column tag is a permanently attached electronic tag device for HPLC columns (≥2.x µm particles) used with Alliance iS and Alliance iS Bio HPLC Systems that are optional. Data from column devices (eCord device or eConnect column tag) can be accessed using the respective system’s console. These smart-column abilities provide a quick overview of column history and can be utilized as a basic troubleshooting tool. Data residing on these devices are automatically imported by and later searchable via Empower CDS.
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