SYNAPT G2 MS/HDMS MALDI Source

Rapid Changeover

All instruments feature a unique, motorized engagement system which enables the rapid and easy changeover between API and MALDI modes of operation. Users exchange sources by first removing the API source and then with a motorized stage, raise the MALDI source before securing it in position. The complete process to change from API acquisition to MALDI Acquisition can typically be achieved in two hours.

The exact mass MALDI-MS/MS data provided by SYNAPT series MS/HDMS instruments provide:

• Improved protein identification and protein sequence coverage
• Characterization of proteins and PTMs using exact MALDI-MS and MS/MS technology
• Significantly improved de novo sequencing compared to post-source decay (PSD) techniques
• Sensitive, accurate analysis of polymers, glycans, small molecules, and oligonucleotides
• Metabolite identification and profiling
• Biomarker discovery
• Molecular imaging

The MALDI SYNAPT G2 HDMS series instruments with their additional ion mobility separation capability also offers the possibility of ground breaking experimentation in new areas of research.

LC-MALDI

Specific proteins from within complex mixtures that are only ionized by MALDI, can also be accommodated by first carrying out liquid chromatography separations followed by MS analyses.

An off-line LC separation is carried out and a robotic spotting device is used to spot the eluting LC fractions directly onto a MALDI target plate. This is then introduced into the instrument for MALDI analysis.

This procedure is particularly effective for protein identification using peptide mass fingerprinting where low-abundance and high-abundance proteins are mixed together.

With other traditional methods low-abundance proteins are undetected due to ion suppression effects, instrumental dynamic range limitations and chemical noise interferences.

LC MALDI analyses provide:

• Chromatographic separation spatially preserved on the MALDI target
• Generation of high sensitivity, interference-free, off-line LC-MS and LC-MS/MS data by MALDI
• Peptide mass mapping of low-abundance proteins in mixtures containing high-abundance proteins

MALDI MS Imaging

The MALDI source accessory offers you the possibility of carrying out MALDI MS imaging on MALDI SYNAPT™ G2 series MS/HDMS™ systems. This can be further extended to include novel ion mobility studies with MALDI and MS imaging for the SYNAPT G2 HDMS series instruments.

MALDI imaging has the potential to enable misfolded proteins in tissue samples to be detected and so enhance the understanding of various human diseases.

The MALDI G2 SYNAPT series MS and HDMS sources all include as standard a microscope plate holder assembly enabling the direct introduction of conventional microscope plates.

Furthermore, MALDI SYNAPT G2, MALDI SYNAPT G2-S, and MALDI SYNAPT G2-Si series instruments are equipped as standard with a 2.5 KHz high repetition rate solid state laser system, offering reduced ablation times for MALDI Imaging experiments. Average laser spot size has been reduced to 60 µm in the current MALDI Source design.

Instrument Compatibility

Installation of the MALDI source is only recommended for the following instruments when already equipped with at least an 8,000 amu mass range quadrupole analyzer. However, there are some small molecule MALDI applications where a 4,000 amu quadrupole analyzer is satisfactory. The MALDI source can also be added to SYNAPT G2 series MS/HDMS instruments already equipped with the 32,000 amu mass range quadrupole analyser.

• SYNAPT G2 MS & SYNAPT G2 HDMS (2.5 KHz laser)
• SYNAPT G2-S MS & SYNAPT G2-S HDMS (2.5 KHz laser)
• SYNAPT G2-Si MS & SYNAPT G2-Si HDMS (2.5 KHz laser)

On-site conversion of 4,000 amu quadrupole analysers to 8,000 amu mass range is also available for all SYNAPT series instruments.