5 Rules of Scaling LC Purification Rule 3. Always Be Consistent with Column Volume

Library Number:
LITR135135121
Part Number:
720008358EN
Author(s):
Jo-Ann Jablonski
Source:
Waters
Content Type:
Literature
Content Subtype:
Brochures

This Mini Article describes about the scaling up gradient separations in liquid chromatography (LC) for molecular target purification and also explains the calculation of column volumes, both for empty and packed columns, and underscores the importance of using consistent volume equations throughout an experiment to ensure reliable results.


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