Become a Peptide Pro: Peptide Bioanalysis

Check out the full blog series below:

• Where Did My Sample Go? Peptides and proteins are especially prone to non-specific binding (NSB) or adsorption to solid surfaces. These surfaces could be pipette tips, sample storage containers, or injection needles. Let’s examine the consequence of non-specific binding on sample analysis, including low recovery, high variability, poor sensitivity and insufficient linear dynamic range.
• Lost Samples in the Container: Non-specific Binding and the Impact of Blocking Agents: When we think of good sample prep and handling, we emphasize the sample purity, concentration, volume, etc. but often overlook the importance of sample recovery during storage.
• Sample Prep before LC-MS Quantification of Peptides and Proteins: Learn more into why your choice of sample container is so important in minimizing non-specific binding and making sure you don’t lose your precious sample. It is a bit like choosing the right hotel room for your vacation or holiday!
• What Do Great Vacations and Peptide Bioanalysis Have in Common? Often bioanalysts evaluate the sensitivity, accuracy, and precision of data from a high performance triple quadrupole mass spectrometer as a marker of assay performance, but how often is the influence of good sample handling, preparation, and separation overlooked?
• 5 Peptide Bioanalysis Sample Preparation Challenges (and how to overcome them!): Explore other sample preparation challenges and how to overcome them.