Column Particle Technologies

Ethylene Bridged Hybrid (BEH) particle technology ensures maximum column performance and longer column lifetimes under all chromatographic conditions. The hybrid particle technology offers many advantages over conventional silica-based particles, including the ability to control silanol activity for better reproducibility, peak shape, and efficiency. The BEH particle is created from two high purity monomers: tetraethoxysilane (TEOS) and bis(triethoxysilyl) ethane (BTEE), which results in highly stable, pH resistant, and mechanically strong particles that set the performance standard for method development. BEH particle technology allows for seamless transfer from analytical to preparative separations.

The advantages of BEH columns include:

• Rugged hybrid organic/inorganic base particle

•  Improved peak shape for bases-low silanol activity

•  Wider operating pH range for method development flexibly

•  Mechanical strength for maximum column efficiency at UHPLC operating pressures

Available Particle Sizes: 1.7, 2.5, 3.5, 5, and 10 µm

Column Chemistries: C₁₈, C₁₈ AX, Shield RP₁₈, C₈, Phenyl, Amide, Z-HILIC, HILIC

The Charged Surface Hybrid (CSH) particle is Waters third generation hybrid particle technology. Based on Waters Ethylene Bridged Hybrid (BEH) particle technology, CSH particles incorporate a low-level surface charge that improves sample loadability and peak asymmetry in low-ionic-strength mobile phases, while maintaining the mechanical and chemical stability inherent in the BEH particle technology.

The advantages of CSH columns include:

• Superior peak shape for basic compounds
• Increased loading capacity
• Rapid column equilibration after changing mobile-phase pH
• Improved batch-to-batch reproducibility
• Exceptional stability at low and high pH
• Seamless transfer between HPLC and UPLC technology platforms

Available Particle Sizes: 1.7, 2.5, 3.5, 5, and 10 µm

Column Chemistries: C₁₈, Phenyl-Hexyl, Fluoro-Phenyl

High pore volume HPLC particles do not possess the mechanical stability necessary to withstand the high pressures inherent in UPLC separations. Waters material scientists addressed this challenge by developing a silica particle designed for high mechanical stability with the appropriate morphology to provide long UPLC column lifetimes and high UPLC efficiencies at high pressures. The 1.8 µm High Strength Silica (HSS) particle is the first and only 100% silica particle designed, tested, and intended for use in applications up to 15,000 psi (1034 bar).

The advantages of HSS columns include:

• High retentivity for polar organic compounds and metabolites
• Balanced retention of polar and hydrophobic analytes

• Mechanical stability at high pressures
• Seamless transfer between HPLC and UPLC technology platforms

Available Particle Sizes: 1.8, 2.5, 3.5, and 5 µm

Column Chemistries: C₁₈, C₁₈SB, T₃, PFP, CN

With advancements in high-performance LC systems designed to drive chromatographic performance, Waters developed solid-core particles that maximize efficiency to drive separation speed, sensitivity, and resolution. CORTECS Columns are designed to provide maximum efficiencies on any LC system. CORTECS 1.6 µm columns provide maximum efficiency on UPLC/UHPLC systems and CORTECS 2.7 µm columns provide maximum utility increasing efficiency on HPLC systems. No matter what the separation challenge, CORTECS solid-core particles can help you achieve your separation goals.

The advantages of Solid-Core columns include:

• Increase column efficiency compared to fully porous particles
• Lower operating backpressures compared to fully porous particles
• Chemistries that have a wide selectivity space for reversed phase methods development

Available Particle Sizes: 1.6 and 2.7 µm

Column Chemistries: C₁₈, C₁₈+, T₃, Shield RP₁₈, C₈, Phenyl, HILIC