Large Molecule Bioanalysis

Large Molecule Bioanalysis

Address the challenges of transitioning to peptide, protein, and oligonucleotide bioanalysis with Waters bespoke chemistries, optimized chromatography, and flexible high-performance mass spectrometry technologies. Waters UPLC-MS/MS large molecule solutions combine sensitivity, reproducibility, and productivity for optimized quantitative performance across the DMPK modality landscape.

Address the challenges of transitioning to peptide, protein, and oligonucleotide bioanalysis with Waters bespoke chemistries, optimized chromatography, and flexible high-performance mass spectrometry technologies. Waters UPLC-MS/MS large molecule solutions combine sensitivity, reproducibility, and productivity for optimized quantitative performance across the DMPK modality landscape.
Learn how to achieve high performance oligonucleotide preparative chromatography by utilizing XBridge Oligonucleotide OBD Prep Columns and efficient method optimization considerations
Learn how to achieve high performance oligonucleotide preparative chromatography by utilizing XBridge Oligonucleotide OBD Prep Columns and efficient method optimization considerations

Overview

Bioanalysis of biopharmaceuticals like peptides, proteins, and oligonucleotides present DMPK scientists with new challenges in sample isolation, separation, detection, and throughput. The complex nature of biopharmaceuticals requires scientists to solve the problems of analyte chromatographic absorption, sample processing, mass spectrometry (MS) method development, and data analysis.

Waters advanced chromatography, sample preparation kits, standards, software, and high sensitivity mass spectrometry solutions are designed to simplify the process of method development and ensure confident routine analysis throughout the drug development pipeline.

Download DMPK Application Notebook

Applications

Oligonucleotides represent an ever-growing proportion of the pharmaceutical pipeline. Learn how Waters comprehensive suite of analytical solutions supports bioanalysis across the analytes of biological therapies in these application notes.

Oligonucleotides represent an ever-growing proportion of the pharmaceutical pipeline. Learn how Waters comprehensive suite of analytical solutions supports bioanalysis across the analytes of biological therapies in these application notes.

Application Notes

Application Notes

Peptides continue to draw attention as potential drug candidates for a wide variety of diseases given their greater efficacy, safety, and tolerability in humans compared to small molecules. Learn about lab-scale purification, characterization, and monitoring and peptides and proteins with these application notes.

Peptides continue to draw attention as potential drug candidates for a wide variety of diseases given their greater efficacy, safety, and tolerability in humans compared to small molecules. Learn about lab-scale purification, characterization, and monitoring and peptides and proteins with these application notes.

Application Notes

Application Notes

eBook: Sample Extraction and LC Tools for Reliable LC-MS Quantitation of Oligonucleotide

Oligonucleotide therapeutics operate at the level of gene transcription and translation and are providing opportunities to address inherited disorders and difficult to treat ailments such as cardiovascular disease. Learn how Waters OligoWorks solid phase extraction (SPE) kits and components help perfect sample preparation for LC-MS-based quantitation of oligonucleotides used in therapeutic delivery.

Oligonucleotide therapeutics operate at the level of gene transcription and translation and are providing opportunities to address inherited disorders and difficult to treat ailments such as cardiovascular disease. Learn how Waters OligoWorks solid phase extraction (SPE) kits and components help perfect sample preparation for LC-MS-based quantitation of oligonucleotides used in therapeutic delivery.
Download eBook
Oligonucleotide illustration

Solutions

Waters UPLC-MS/MS systems deliver absolute performance in separation and sensitivity for bioanalysis of proteins, peptides, and oligonucleotides.

Waters UPLC-MS/MS systems deliver absolute performance in separation and sensitivity for bioanalysis of proteins, peptides, and oligonucleotides.

Confident detection and quantification of large molecule analytes in biological matrices

Confident detection and quantification of large molecule analytes in biological matrices
Xevo TQ Absolute

Expand your analytical capabilities with the Xevo TQ-Absolute. Bringing superior performance and absolute sensitivity for robust quantification of large molecules with our highest-performing compact and sustainable tandem quadrupole mass spectrometer.

  • Oligonucleotide Analysis
  • Peptide and Protein Bioanalysis

Dependable high performance chromatography for large molecules

Dependable high performance chromatography for large molecules
ACQUITY Premier UPLC System

Optimize chromatographic resolution and speed while eliminating concerns of non-specific binding for peptide, protein, and oligonucleotide analytes with Waters ACQUITY Premier UPLC System, enhancing quantitative performance in bioanalysis assays.

  • Oligonucleotide Analysis
  • Peptide and Protein Bioanalysis

Accessible optimized sensitivity for quantitation of large molecules

Accessible optimized sensitivity for quantitation of large molecules
ionKey/MS

Enhance sensitivity for low-volume peptide and protein samples with Waters ionKey Source with ACQUITY M-Class System, delivering the robustness and ease of implementation required for quantitative bioanalytical applications.

  • Oligonucleotide Analysis
  • Peptide and Protein Bioanalysis

Let your LC inlet do the work for your nano- to micro-scale separations

Let your LC inlet do the work for your nano- to micro-scale separations
ACQUITY UPLC M-Class System

Achieve the performance you expect from nano- to microscale separations with the usability of an analytical-scale UPLC system with ACQUITY UPLC M-Class System.

  • Oligonucleotide Analysis
  • Peptide and Protein Bioanalysis

Streamlined confidence for GLP and Non-GLP in vivo bioanalysis.

Streamlined confidence for GLP and Non-GLP in vivo bioanalysis.

Streamline quantitative data acquisition and processing

Streamline quantitative data acquisition and processing
MassLynx Quantitation Applications

Perform quantitative mass spectrometry analysis with MassLynx Quantitation Applications, offering a range of application managers with specialized data processing functionalities, eliminating the need for complex time-consuming manual data review.

  • Oligonucleotide Analysis
  • Peptide and Protein Bioanalysis

Ensure modality versatility in your bioanalysis lab with automation-friendly tailored sample preparation kits, streamlining method development for quantifying proteins, peptides, and oligonucleotides in biofluids.

Ensure modality versatility in your bioanalysis lab with automation-friendly tailored sample preparation kits, streamlining method development for quantifying proteins, peptides, and oligonucleotides in biofluids.

Simplify bioanalysis of biopharmaceuticals 

Simplify bioanalysis of biopharmaceuticals 
OligoWorks Kits

Simplify and accelerate the extraction of oligonucleotide-based drugs and metabolites from biofluids (plasma, urine, tissue homogenates) with OligoWorks SPE Kits, designed to deliver maximum LC-MS sensitivity and repeatability.

  • Oligonucleotide Analysis
  • Peptide and Protein Bioanalysis

Take the complexity out of protein quantification

Take the complexity out of protein quantification
ProteinWorks Kits

Simplify and accelerate protein digestion, streamlining and standardizing complex pre-analytic bioanalysis/DMPK workflows with ProteinWorks eXpress Digest Kits for LC-MS protein quantification in whole or immunopurified plasma.

  • Oligonucleotide Analysis
  • Peptide and Protein Bioanalysis

 Simplify bioanalysis of biopharmaceuticals

 Simplify bioanalysis of biopharmaceuticals
ACQUITY Premier Columns

Develop the most sensitive reliable and high-performance bioanalysis methods and eliminate risks associated with unpredictable chromatography caused by non-specific binding with ACQUITY Premier Columns with MaxPeak High Performance Surfaces (HPS) Technology.

  • Oligonucleotide Analysis
  • Peptide and Protein Bioanalysis

Achieve testing efficiencies and accurate results using streamlined Waters services and support.

Achieve testing efficiencies and accurate results using streamlined Waters services and support.

Your success is just a click away

Your success is just a click away
Waters Global Services

Optimize your laboratory's productivity and success with Waters Global Services. Maintain peak system performance, minimize downtime, address application challenges, and support stringent compliance requirements.

  • Oligonucleotide Analysis
  • Peptide and Protein Bioanalysis

Make science more accessible with Waters Capital

Make science more accessible with Waters Capital
Payment Solutions

Maximize your lab resources and minimize risk with payment options from Waters Capital, which includes innovative solutions to upgrade aging instruments, customized support, and flexible options to bundle your complete laboratory solution in one easy monthly payment everything you need to advance your science.

  • Oligonucleotide Analysis
  • Peptide and Protein Bioanalysis

The data speaks for itself

The data speaks for itself
Demonstration of improved out-of-the-box chromatographic performance (oligonucleotide recovery) for the GEM91 using the ACQUITY Premier Oligonucleotide C18 vs standard ACQUITY UPLC Oligonucleotide BEH C18 Column.
Representative chromatograms of lowest calibration standards of oligonucleotides analyzed in plasma by ACQUITY Premier/ Xevo TQ Absolute.
MRM optimization data acquired on the Xevo TQ-XS and imported into Skyline.
Mitigation of hydrophobic NSB to collection vessels using QuanRecovery™ with MaxPeak HPS 96-well Sample Collection plate, demonstrating improved MS peak response vs standard polypropylene plates.

Webinars and Resources

  • Journal Citations

Practical Applications of Integrated Microfluidics for Peptide Quantification

Practical Applications of Integrated Microfluidics for Peptide Quantification
Download
  • Infographic

Large Molecule Analysis by LC-MS–Survey Infographic

Large Molecule Analysis by LC-MS–Survey Infographic
Download
  • Application Notebook

Peptide and Protein Bioanalysis 

Peptide and Protein Bioanalysis 
Download
  • Application Notebook

ProteinWorks

ProteinWorks
Download
  • Application Notebook

Versatile LC-MS/MS Solutions for the Evolving Bioanalysis Landscape

Versatile LC-MS/MS Solutions for the Evolving Bioanalysis Landscape
Download
  • eBook

Quantifying Oligonucleotides eBook

Quantifying Oligonucleotides eBook
Download
  • Brochure

ProteinWorks

ProteinWorks
Download

Related

Quantitative Bioanalysis
Agilely support the progression of drug candidates throughout the drug development pipeline with Waters versatile quantitative bioanalysis solutions.

Emerging Modalities in DMPK
Develop robust and sensitive bioanalytical methods for DMPK testing with Waters versatile, high-performance UPLC-MS/MS bioanalysis solutions. View our DMPK solutions to find tools and techniques to support the bioanalysis of novel modalities.

Small Molecule Bioanalysis
Accurately perform small molecule bioanalysis of drug therapeutics with Waters UPLC-MS/MS solutions, designed to maximize throughput and minimize non-specific absorption.

Learn more about Large Molecule Bioanalysis Solutions.

Learn more about Large Molecule Bioanalysis Solutions.
Learn how to achieve high performance oligonucleotide preparative chromatography by utilizing XBridge Oligonucleotide OBD Prep Columns and efficient method optimization considerations
Back To Top Back To Top